24 Hours of Stem Cells

TaqMan® hPSC Scorecard™ Panel and data analysis software brochure

Overview of the TaqMan® hPSC Scorecard™ Panel and data analysis software. Learn how the assay works and compatibile instruments.
http://vshow.on24.com/vshow/life/content/707908

ProFlex™ PCR System Technical Specifications flyer

Compare technical specifications of the four different blocks offered for the ProFlex™ PCR System.
http://vshow.on24.com/vshow/life/content/707848

GeneArt® Strings™ DNA Fragments product bulletin

Learn more about GeneArt® Strings™ DNA fragments product bulletin - custom-made double-stranded DNA, ready to clone.
http://vshow.on24.com/vshow/life/content/717357

Unboxing ProFlex™ PCR System

Learn how to unbox and quickly setup your new ProFlex™ PCR System.
http://vshow.on24.com/vshow/life/content/719253

Modeling neurodegenerative disease - Research Highlight: Induced pluripotent stem cell (iPSC) generation with CytoTune® reprogramming kit

Modeling neurodegenerative disease - Stem cells help develop cellular models for understanding Parkinson’s disease. Series 1 highlight: Induced pluripotent stem cell (iPSC) generation using the CytoTune®-iPS Sendai Reprogramming Kit.
http://vshow.on24.com/vshow/life/content/719281

Resource

http://vshow.on24.com/vshow/life/content/711762

Resource

http://vshow.on24.com/vshow/life/content/711869

Pluripotent stem cell protocols

View a selection of protocols for culturing, reprogramming, differentiating, and characterizing PSCs.
http://vshow.on24.com/vshow/life/content/720763

Resource

http://vshow.on24.com/vshow/life/content/711867

Basic techniques in autophagy research

This webinar will introduce you to a series of analytical tools and techniques to help you identify and interrogate key features of autophagy. Topics to be covered include: - Tips and tricks for selecting the right tools and achieving the best results - Fluorescent proteins and antibodies used to analyze both live and fixed cells - Analysis steps using a variety of multiplexing options, with quantitative methods for image analysis or fluorescence intensity measurement
http://vshow.on24.com/vshow/life/content/712250

An introduction to immunofluorescent staining of cultured cells

Life Technologies Technical Support presents an introduction to immunofluorescent staining of culture cells.
http://vshow.on24.com/vshow/life/content/710572

Resource

http://vshow.on24.com/vshow/life/content/711787

Veriti® Thermal Cycler video

Learn more about the Veriti® Thermal Cycler. View produve overview, user interface, and more.
http://vshow.on24.com/vshow/life/content/719259

Development of high quality MSC and NSC for human disease research

Presented ISSCR 2013
http://vshow.on24.com/vshow/life/content/710044

Imaging and flow cytometry reagents from Molecular Probes®

Learn more about cell health reagents for imaging and flow cytometry
http://vshow.on24.com/vshow/life/content/710533

Cell biology product and resource guide, 2013

Complete list of all cell biology products and services offered by Life Technologies. Don't forget to check out the research areas for products tailored for your research.
http://vshow.on24.com/vshow/life/content/710041

Neural cell culture sourcebook

Comparison of neural cell culture tools for primary and stem cell applications.
http://vshow.on24.com/vshow/life/content/710038

A comparison of basic immunofluorescent labeling strategies

Life Technologies technical support presents a comparison of basic immunofluorescent labeling strategies.
http://vshow.on24.com/vshow/life/content/714208

Vector NTI® Express Sequence Analysis & Design Software

The Vector NTI® Express software package is the complete bioinformatics solution. Completely redesigned to be faster, more intuitive and with a dedicated Mac OS® X version Vector NTI® Express Software retains the core, trusted tools of Vector NTI Advance® Software with a brand new interface. This completely integrated suite of sequence analysis & design tools helps you manage, view, analyze, transform, share, and publicize diverse types of molecular biology data, all within one graphically rich analysis environment.
http://vshow.on24.com/vshow/life/content/719244

A comparison of basic immunofluorescent labeling strategies

Life Technologies technical support presents a comparison of basic immunofluorescent labeling strategies
http://vshow.on24.com/vshow/life/content/710574

Resource

http://vshow.on24.com/vshow/life/content/710481

TaqMan® Assays for rapid assessment of genome, epigenome, and quality of integration-free iPSCs

Presented ISSCR 2013
http://vshow.on24.com/vshow/life/content/710055

Ready Probes Flyer: 即用型Molecular probe产品介绍

Ready Probes Flyer: 即用型Molecular probe产品介绍
http://vshow.on24.com/vshow/life/content/722540

Gibco® neurobiology protocol handbook

Over 20 protocols for neurobiolgy research. Includes protocols for differentiation and culture of NSCs.
http://vshow.on24.com/vshow/life/content/711971

Xeno-free culture systems for pluripotent stem cells

Presented ISSCR 2012
http://vshow.on24.com/vshow/life/content/710046

ON24_ExHallLogo_90x40

ON24_ExHallLogo_90x40-1F01.png
http://vshow.on24.com/vshow/life/content/707348

Fluorophore selection in experimental design

The choice of fluorophore is one of the first important decisions to make in developing an experiment. Fluorophores are compounds that emit light when excited with another, lower, wavelength, but there are many different types. This webinar will cover: - How to choose the best organic dye for an assay - Describe quantum dots and how they compare to other dyes - When to use a phycobiliprotein like R-PE or APC - When to use fluorescent proteins, like GFP - Choosing a suitable dye to match your instrument In addition, we will explore the basic characteristics, strengths, and weaknesses of the various fluors to help you choose and develop the best assay for your needs.
http://vshow.on24.com/vshow/life/content/712254

Resource

http://vshow.on24.com/vshow/life/content/711744

How to use Gibco® PSC Neural Induction Medium

This video will show you how Gibco® PSC Neural Induction Medium generates up to 20 million neural stem cells from pluripotent stem cells in 7 days.
http://vshow.on24.com/vshow/life/content/719268

Essential 8™ and Essential 6™ media system flyer

When used together, Essential 8™ and Essential 6™ media let you move easily between reprogramming, expansion, and differentiation of pluripotent stem cells (PSCs)
http://vshow.on24.com/vshow/life/content/707910

Advances in stem cell research: Cell reprogramming with episomal iPSC reprogramming vectors

Dr. Emile Nuwaysir, Chief Operating Officer of Cellular Dynamics International, discusses the integration-free and viral-free iPSC generation using the Invitrogen Episomal iPSC Reprogramming Vectors.
http://vshow.on24.com/vshow/life/content/719284

NIM protocal: 神经诱导培养基实验操作方案

NIM protocal: 神经诱导培养基实验操作方案
http://vshow.on24.com/vshow/life/content/722522

GeneArt® Precision TALs flyer

GeneArt® Precision TALs provide custom DNA-binding proteins for accurate DNA targeting and precise genome editing. Other technologies limit your choice of targets, but with GeneArt® Precision TALs you can target any locus in the genome—and more accurate genome engineering allows you to develop better solutions.
http://vshow.on24.com/vshow/life/content/717355

Ph.Diddy is on the scene: 屏幕上的Diddy博士

Ph.Diddy is on the scene: 屏幕上的Diddy博士
http://vshow.on24.com/vshow/life/content/722539

Final Agenda

http://vshow.on24.com/vshow/life/content/722407

Click-iT Edu Flyer: Click-iT Edu细胞增殖检测产品介绍

Click-iT Edu Flyer: Click-iT Edu细胞增殖检测产品介绍
http://vshow.on24.com/vshow/life/content/722541

ProFlex™ PCR System made easy

Learn how to run your ProFlex™ PCR System quickly in a few simple steps.
http://vshow.on24.com/vshow/life/content/719254

Ambion® Single Cell-to-CT™ Kit brochure

Learn more about single cell technology available from Life Technologies. The Ambion® Single Cell-to-CT™ Kit provides a complete validated workflow for gene expression analysis for samples containing between 1 and 10 cells.
http://vshow.on24.com/vshow/life/content/722409

Neural cell culture sourcebook

Options for neural cell culture - including leading products like B-27® Supplements and Neurobasal® Medium.
http://vshow.on24.com/vshow/life/content/711968

Exosomes产品介绍单页

Exosomes产品介绍单页
http://vshow.on24.com/vshow/life/content/722536

LifeLab™ pluripotent stem cell workshops flyer

Learn more about LifeLab™ stem cell training courses offered by Life Technologies. LifeLab™ stem cell research centers provide training for culturing and characterizing hESCs and iPSCs, as well as reprogramming techniques for the derivation of iPSCs. View dates for available 2014 workshops now.
http://vshow.on24.com/vshow/life/content/720822

How to Prepare Vitronectin Coated Plate:如何准备玻连蛋白包被的培养皿

How to Prepare Vitronectin Coated Plate:如何准备玻连蛋白包被的培养皿
http://vshow.on24.com/vshow/life/content/722529

CytoTune®-iPS 2.0 Reprogramming Kit frequently asked questions

Find the answers to commonly asked questions regarding CytoTune® reprogramming technology.
http://vshow.on24.com/vshow/life/content/711812

ProFlex™ PCR System Presentation

Learn more about the ProFlex™ PCR System by viewing this slide presentation.
http://vshow.on24.com/vshow/life/content/707861

Modeling neurodegenerative disease - Research Highlight: Analysis using the TaqMan® hPSC Scorecard™ Panel

Modeling neurodegenerative disease - Stem cells help develop cellular models for understanding Parkinson’s disease. Series 1 highlight: Analysis using the TaqMan® hPSC Scorecard™ Panel.
http://vshow.on24.com/vshow/life/content/714250

Matrices sourcebook - Extracellular matrices products guide

Learn more about extracellular matrices products available from Life Technologies.
http://vshow.on24.com/vshow/life/content/710040

Stem cell products and services resource guide

View workflow examples, protocol overviews, and details about products and services available for stem cell research
http://vshow.on24.com/vshow/life/content/710056

Isolate RNA with the PureLink™ RNA Mini Kit

The PureLink™ RNA Mini Kit provides rapid purification of total RNA from a wide range of cell and tissue types, including animal, plant, and yeast cells, bacteria, and blood. PureLink™ technology combines guanidine-isothiocyanate lysis with the speed, purity, and ease of use of silica-membrane purification. The safe and easy procedure can be completed in less than 20 minutes without the need for hazardous phenol/chloroform extraction, CsCl centrifugation, or LiCl or alcohol precipitation. High-quality purified RNA can be obtained from mini- to midi-prep amounts of starting material, with minimal genomic DNA contamination. Purified RNA is ready to be used in downstream applications such as RT-PCR, qRT-PCR, northern blots, cDNA synthesis, and nuclease protection assays.
http://vshow.on24.com/vshow/life/content/719806

The Complete Exosome Workflow Solution: From Isolation to Characterization of RNA Cargo

2013 scientific paper
http://vshow.on24.com/vshow/life/content/710269

ON24_Logo_307x146

ON24_Logo_307x146-AA1E.png
http://vshow.on24.com/vshow/life/content/707349

Reagents, kits, and instrument options for advanced analysis of exosomes

2013 Scientific poster
http://vshow.on24.com/vshow/life/content/710284

Frequently asked questions for Episomal iPSC Reprogramming Vectors

Answers to commonly asked questions regarding Episomal iPSC Reprogramming Vectors
http://vshow.on24.com/vshow/life/content/720766

Exosome Tracing Application Note

Labeling exosomal RNA and membrane components using flourescent dyes
http://vshow.on24.com/vshow/life/content/707734

Methods for the extraction and RNA profiling of exosomes

2013 scientific paper
http://vshow.on24.com/vshow/life/content/710331

An introduction to immunofluorescent staining of cultured cells

Life Technologies Technical Support provides an introduction to immunofluorescent staining of culture cells.
http://vshow.on24.com/vshow/life/content/714209

Modeling neurodegenerative disease - Parkinson’s disease cell models – White paper series 1

Modeling neurodegenerative disease - Stem cells help develop cellular models for understanding Parkinson’s disease - White paper series 1.
http://vshow.on24.com/vshow/life/content/719293

Visualizing internalized cargo acidification in live cells with novel pH-sensing fluorescent dyes

2013 scientific poster
http://vshow.on24.com/vshow/life/content/710487

Synthetic biology brochure

Learn more about products and services offered to create a complete solution for synthetic biology research & industry needs.
http://vshow.on24.com/vshow/life/content/717360

Stem cell products and services resource guide

View workflow examples, protocol overviews, and details about products and services available for stem cell research.
http://vshow.on24.com/vshow/life/content/720767

EVOS® Image Gallery

Video of images taking using EVOS® cell imaging systems.
http://vshow.on24.com/vshow/life/content/710628

Efficient and rapid derivation of primitive neural stem cells and generation of brain subtype neurons from human pluripotent stem cells

Human pluripotent stem cells (hPSCs), including human embryonic stem cells and human induced pluripotent stem cells, are unique cell sources for disease modeling, drug discovery screens, and cell therapy applications. The first step in producing neural lineages from hPSCs is the generation of neural stem cells (NSCs). Current methods of NSC derivation involve the time-consuming, labor-intensive steps of an embryoid body generation or coculture with stromal cell lines that result in low-efficiency derivation of NSCs. In this study, we report a highly efficient serum-free pluripotent stem cell neural induction medium that can induce hPSCs into primitive NSCs (pNSCs) in 7 days, obviating the need for time-consuming, laborious embryoid body generation or rosette picking. The pNSCs expressed the neural stem cell markers Pax6, Sox1, Sox2, and Nestin; were negative for Oct4; could be expanded for multiple passages; and could be differentiated into neurons, astrocytes, and oligodendrocytes, in addition to the brain region-specific neuronal subtypes GABAergic, dopaminergic, and motor neurons. Global gene expression of the transcripts of pNSCs was comparable to that of rosette-derived and human fetal-derived NSCs. This work demonstrates an efficient method to generate expandable pNSCs, which can be further differentiated into central nervous system neurons and glia with temporal, spatial, and positional cues of brain regional heterogeneity. This method of pNSC derivation sets the stage for the scalable production of clinically relevant neural cells for cell therapy applications in good manufacturing. practice conditions.
http://vshow.on24.com/vshow/life/content/720762

TAL effectors tool box for precision genome editing

Designer TAL effectors are versatile not only in their function, but have also been shown to work in a variety of host systems. At Life Technologies, we can engineer TAL binding proteins through our GeneArt® Precision TAL offerings and fuse these proteins to any designated effector domain, thereby targeting any genomic sequence or locus with high precision. Watch this presentation for an introduction to designer TAL effectors and their applications for targeted gene editing.
http://vshow.on24.com/vshow/life/content/719235

Genome editing of iPSCs from Parkinson’s disease patients using TAL technology

During this presentation we examine genetic engineering of iPSCs derived from Parkinson’s disease patient samples, which provide model systems to study disease mechanisms in cell types not previously available. Next-generation tools for stem cell engineering, GeneArt® Precision TALs offer an extremely efficient method for targeting and regulating expression at defined sequences within the genome, enabling you to more accurately engineer and study gene function to develop better stem cell models.
http://vshow.on24.com/vshow/life/content/719234

What is synthetic biology?

Find out what synthetic biology is, how it can improve life and the tools Life Technologies™ can provide.
http://vshow.on24.com/vshow/life/content/719245

Using time-lapse imaging with the EVOS® FL Auto Cell Imaging System

Application note
http://vshow.on24.com/vshow/life/content/710561

Stem Cell Brochure 干细胞产品手册

Stem Cell Brochure 干细胞产品手册
http://vshow.on24.com/vshow/life/content/722516

EVOS® cell imaging systems brochure

Learn more about the product family of EVOS® cell imaging systems: Smarter systems, easier cell imaging, faster results. EVOS® cell imaging systems include: EVOS® XL Core, EVOS® XL, EVOS® FLoid®, EVOS® FL, and the EVOS® FL Auto.
http://vshow.on24.com/vshow/life/content/710493

Image tiling and stitching using the EVOS® FL Auto Cell Imaging System

Application note
http://vshow.on24.com/vshow/life/content/710564

Stem cell products and services resource guide

View workflow examples, protocol overviews, and details about products and services available for stem cell research
http://vshow.on24.com/vshow/life/content/711781

Resource

http://vshow.on24.com/vshow/life/content/711796

Creating integration-free iPSCs and NSCs to model Parkinson’s disease by Dr. Birgitt Schuele

Watch the ISSCR 2013 presentation by Dr. Birgitt Schuele of the Parkinson's Institute. Generating Parkinson's Disease models using Footprint-free Reprogramming and a Novel Method for NSC Differentiation The ability to generate iPSC-derived models that recapitulate phenotypes associated with Parkinson's Disease holds promise to accelerate understanding of the disease with a goal of finding a cure. By using non-integrating Sendai-virus (SeV) based CytoTune® reprogramming technologies, we have created patient-derived iPSCs of known genetic backgrounds, and used a novel method to create neural stem cells (NSCs) without going through the laborious process of EB formation followed by rosette isolation.
http://vshow.on24.com/vshow/life/content/719283

Pluripotent stem cell protocols

View a selection of protocols for culturing, reprogramming, differentiating, and characterizing PSCs.
http://vshow.on24.com/vshow/life/content/720759

ON24_CompanyLogo_309x152

ON24_CompanyLogo_309x152-D64C.png
http://vshow.on24.com/vshow/life/content/707347

NIM Medium flyer 神经诱导培养基单页

NIM Medium flyer 神经诱导培养基单页
http://vshow.on24.com/vshow/life/content/722520

How to use the hPSC Scorecard™ data analysis software

This video will show you how to analyze your TaqMan® hPSC Scorecard™ Panel data using the hPSC Scorecard™ Analysis Software.
http://vshow.on24.com/vshow/life/content/714114

Webinar: Exosomes 2013: New perspectives, tools, and technologies

Webinar discussing strategies and new tools for exosome isolation and analysis
http://vshow.on24.com/vshow/life/content/710278

How to Prepare Essential 8™ Medium:  如何准备Essential 8™ 培养基

How to Prepare Essential 8™ Medium:  如何准备Essential 8™ 培养基
http://vshow.on24.com/vshow/life/content/722528

LifeLab™ pluripotent stem cell workshops flyer

Learn more about LifeLab™ stem cell training courses offered by Life Technologies. LifeLab™ stem cell research centers provide training for culturing and characterizing hESCs and iPSCs, as well as reprogramming techniques for the derivation of iPSCs. View dates for available 2014 workshops now.
http://vshow.on24.com/vshow/life/content/720815

Robust cDNA synthesis with SuperScript® III Reverse Transcriptase

Please join us for this live interactive webinar featuring Life Technologies scientists as they discuss: Key attributes of Reverse Transcription, What is a Reverse Trancriptase, Key Applications for cDNA synthesis and SuperScript® VILO™ Master Mix
http://vshow.on24.com/vshow/life/content/711774

Frequently asked questions for the TaqMan® hPSC Scorecard™ Panel

Answers to commonly asked questions regarding the new TaqMan® hPSC Scorecard™ Panel.
http://vshow.on24.com/vshow/life/content/720752

Dr. Ron Hart discusses MicroRNA and Epigenetic Regulation of Neural Stem Cell Differentiations

Dr. Ron Hart, Professor of Cell Biology & Neuroscience at Rutgers University, presents work on the identification of hundreds of novel microRNAs in human embryonic stem cells by deep sequencing and microRNA and epigenetic regulation of neural stem cell differentiation.
http://vshow.on24.com/vshow/life/content/719274

PCR optimization webinar

Review this on-demand webinar for PCR research.
http://vshow.on24.com/vshow/life/content/711786

How to Prepare Vitronectin Coated Plate:如何准备玻连蛋白包被的培养皿

How to Prepare Vitronectin Coated Plate:如何准备玻连蛋白包被的培养皿
http://vshow.on24.com/vshow/life/content/722530

Essential 8™ and Essential 6™ media system flyer

When used together, Essential 8™ and Essential 6™ media let you move easily between reprogramming, expansion, and differentiation of pluripotent stem cells (PSCs).
http://vshow.on24.com/vshow/life/content/711783

PCR essentials brochure

A Guide to PCR Essentials - Life Technologies offers products to help you achieve success in endpoint PCR for virtually any application. With trusted Applied Biosystems® thermal cyclers and MicroAmp® plastic disposables, AmpliTaq® and AmpliTaq Gold® enzymes, and industry-leading SuperScript® III and Platinum® Taq enzymes, you’ll find the tools you need to help you achieve PCR success.
http://vshow.on24.com/vshow/life/content/711421

ExosomesTalk

A place to ask questions and exchange exosomes-related info
http://vshow.on24.com/vshow/life/content/710265

How to prepare Essential 8™ Medium for pluripotent stem cell culture

This video will show you how to prepare complete Essential 8™ Medium for the consistent, robust, cost-effective feeder-free culture of PSCs.
http://vshow.on24.com/vshow/life/content/717361

SuperScript® III Reverse Transcriptase

The SuperScript® Reverse Transcriptases continue to evolve
http://vshow.on24.com/vshow/life/content/711749

Introduction to fast PCR

Learn what is Fast PCR and how you can design your experiment to acheive faster results
http://vshow.on24.com/vshow/life/content/711788

LifeLab™ pluripotent stem cell workshops flyer

Learn more about LifeLab™ stem cell training courses offered by Life Technologies. LifeLab™ stem cell research centers provide training for culturing and characterizing hESCs and iPSCs, as well as reprogramming techniques for the derivation of iPSCs. View dates for available 2014 workshops now.
http://vshow.on24.com/vshow/life/content/720817

Resource

http://vshow.on24.com/vshow/life/content/711853

The Evolution of PSC culture media, ISSCR 2012

Human induced pluripotent stem cells (iPSCs) provide the potential for generating large numbers of wild type and disease iPSC lines to advance discoveries in developmental biology and disease research. Two main challenges in expanding iPSCs are reproducibility and the cost of the culture system, particularly for larger-scale studies. Here Dr. Rupa Shevde discusses the evolution of pluripotent stem cell culture media, from KnockOut™ Serum Replacement on feeders to current feeder-free solutions and provides detailed information on Essential 8™ Medium, a defined feeder-free culture system that provides only the essential components necessary for optimal iPSC culture and expansion, improving reproducibility and differentiation potential while reducing cost.
http://vshow.on24.com/vshow/life/content/717365

How does Sendai Virus reprogramming cells? 仙台病毒如何重编程细胞

How does Sendai Virus reprogramming cells? 仙台病毒如何重编程细胞
http://vshow.on24.com/vshow/life/content/722524

Frequently asked questions for Essential 8™ Medium and Vitronectin

Answers to commonly asked questions regarding Essential 8™ Medium and Vitronectin
http://vshow.on24.com/vshow/life/content/720760

Resource

http://vshow.on24.com/vshow/life/content/711778

TaqMan® human pluripotent stem cell Scorecard™ Panel: a new standard for hPSC characterization

The TaqMan® hPSC Scorecard™ Panel has been designed to accurately evaluate pluripotency, predict trilineage differentiation potential and compare results against a reference standard. Join Dr. Shevde as she provides an overview of this new technology for stem cell characterization.
http://vshow.on24.com/vshow/life/content/710062

Stem Cell Product and Services

http://vshow.on24.com/vshow/life/content/721647

Cell Imaging Toolbox and Promotion for China: Cell Imaging工具指南和优惠活动

Cell Imaging Toolbox and Promotion for China: Cell Imaging工具指南和优惠活动
http://vshow.on24.com/vshow/life/content/722542

Resource

http://vshow.on24.com/vshow/life/content/710370

Creating integration-free iPSCs and NSCs to model Parkinson’s disease by Dr. Birgitt Schuele

Watch the ISSCR 2013 presentation by Dr. Birgitt Schuele of the Parkinson's Institute. Generating Parkinson's Disease models using Footprint-free Reprogramming and a Novel Method for NSC Differentiation. The ability to generate iPSC-derived models that recapitulate phenotypes associated with Parkinson's Disease holds promise to accelerate understanding of the disease with a goal of finding a cure. By using non-integrating Sendai-virus (SeV) based CytoTune® reprogramming technologies, we have created patient-derived iPSCs of known genetic backgrounds, and used a novel method to create neural stem cells (NSCs) without going through the laborious process of EB formation followed by rosette isolation.
http://vshow.on24.com/vshow/life/content/719272

A comparison of basic immunofluorescent labeling strategies

In this webinar we compare different immunofluorescent labeling strategies, exploring the pros and cons of each method. Viewers learn when the use of a direct conjugate is appropriate and when amplification techniques can be utilized. We also present a simple decision tree to aid in determining the best method for each situation.
http://vshow.on24.com/vshow/life/content/712255

GeneArt® Gene Synthesis Services brochure

Whether you need industry-leading gene synthesis services or optimized protein expression, or want to outsource the entire process from gene synthesis to protein production, this brochure outlines GeneArt® services to help you succeed.
http://vshow.on24.com/vshow/life/content/717353

Veriti™ Dx Thermal Cycler brochure

Learn more about the Veriti™ Dx Thermal Cycler.
http://vshow.on24.com/vshow/life/content/707850

Imaging and flow cytometry reagents from Molecular Probes®

Learn more about cell health reagents for imaging and flow cytometry.
http://vshow.on24.com/vshow/life/content/714210

ON24 Scheduled Chat User Guide

http://vshow.on24.com/vshow/life/content/694853

Qubit® 2.0 Fluorometer in Action

The Qubit® 2.0 Fluorometer quantitates DNA, RNA, and protein with unprecedented accuracy, sensitivity, and simplicity. It is designed for molecular biology labs that work with: • Precious samples that are rare or difficult to process • Applications requiring precise measurement such as real-time PCR The Qubit® 2.0 Fluorometer utilizes specifically designed fluorometric technology using Molecular Probes® dyes. These fluorescent dyes emit signals ONLY when bound to specific target molecules, even in the presence of free nucleotides or degraded nucleic acids. Qubit® fluorometric quantitation provides the most specific and sensitive DNA and RNA quantitation available, even at low concentrations
http://vshow.on24.com/vshow/life/content/719811

SuperScript® VILO™ cDNA synthesis kit

The Qubit® 2.0 Fluorometer quantitates DNA, RNA, and protein with unprecedented accuracy, sensitivity, and simplicity. It is designed for molecular biology labs that work with precious samples that are rare or difficult to process or applications requiring precise measurement such as real-time PCR. More accurate than UV absorbance readings, Higher sensitivity - uses as little as 1 µL of sample, An intuitive, integrated system - sophisticated quantitation in 5 minutes or less
http://vshow.on24.com/vshow/life/content/719810

Essential 6™与Essential 8™培养基单页

Essential 6™与Essential 8™培养基单页
http://vshow.on24.com/vshow/life/content/722521

Cell reprogramming tools - Products and services for non-integrating reprogramming

Compare reprogramming technologies and services
http://vshow.on24.com/vshow/life/content/711782

Alkaline Phosphatase Live Stain: 3 steps to successful staining

Troubleshooting video recommended for viewing before using the Alkaline Phosphatase Live Stain. Shows how to successfully complete the 3 key areas of the protocol that can impact staining.
http://vshow.on24.com/vshow/life/content/714118

Cell Health and Cytotoxicity Poster: 细胞健康和细胞毒性海报

Cell Health and Cytotoxicity Poster: 细胞健康和细胞毒性海报
http://vshow.on24.com/vshow/life/content/722543

Cell Structure Poster: 细胞结构海报

Cell Structure Poster: 细胞结构海报
http://vshow.on24.com/vshow/life/content/722544

MicroAmp® EnduraPlate™ optical microplates flyer

Learn more about MicroAmp® EnduraPlate™ optical microplates - a new optical microplate for higherthroughput genetic analysis applications.
http://vshow.on24.com/vshow/life/content/707849

LifeLab™ pluripotent stem cell workshops flyer

Learn more about LifeLab™ stem cell training courses offered by Life Technologies. LifeLab™ stem cell research centers provide training for culturing and characterizing hESCs and iPSCs, as well as reprogramming techniques for the derivation of iPSCs. View dates for available 2014 workshops now.
http://vshow.on24.com/vshow/life/content/720814

Adding Partner Ref Parameter to Registration URLs for Tracking Campaigns

http://vshow.on24.com/vshow/life/content/694854

Resource

http://vshow.on24.com/vshow/life/content/711755

Ph.Diddy at the Conference:会议上的Diddy博士

Ph.Diddy at the Conference:会议上的Diddy博士
http://vshow.on24.com/vshow/life/content/722538

PCR enzymes brochure

Learn more about Platinum® Taq DNA Polymerase and SuperScript® Reverse Transcriptase, two essential enzymes, so you don’t have to compromise your research.
http://vshow.on24.com/vshow/life/content/711422

How does Sendai virus reprogram cells?

This video demonstrates how Sendai virus, found in the Cytotune®-iPS Sendai Reprogramming Kit, reprograms somatic cells to generate induced Pluripotent Stem Cells (iPSCs).
http://vshow.on24.com/vshow/life/content/719282

LifeLab™ pluripotent stem cell workshops flyer

Learn more about LifeLab™ stem cell training courses offered by Life Technologies. LifeLab™ stem cell research centers provide training for culturing and characterizing hESCs and iPSCs, as well as reprogramming techniques for the derivation of iPSCs. View dates for available 2014 workshops now.
http://vshow.on24.com/vshow/life/content/720813

The "What If" is Now: Introducing the new ProFlex™ PCR System

Learn how the ProFlex PCR System (http://Lifetechnologies.com/proflexpcr) can help your PCR become more flexible, convenient and accessible.
http://vshow.on24.com/vshow/life/content/719256

GeneArt® CRISPR Nuclease Vector Kits flyer

Learn more about GeneArt® CRISPR Nuclease Vector Kits.
http://vshow.on24.com/vshow/life/content/715941

BIOPROBES® 69: Journal of cell biology applications

December 2013 issue
http://vshow.on24.com/vshow/life/content/710554

Novel markers to distinguish pluripotent and nonpluripotent cells

Presented ISSCR 2013
http://vshow.on24.com/vshow/life/content/710059

Generation of transgene-free iPSCs from Parkinson’s disease patients

Presented ISSCR 2013
http://vshow.on24.com/vshow/life/content/711784

Utilizing advanced cryopreservation techniques to produce assay ready primary mouse neurons

Presented SFN 2013
http://vshow.on24.com/vshow/life/content/716632

Basics of flow cytometry, Part II: Compensation

This presentation provides an overview of basic fluorochromes used in flow cytometry. Topic includes: the principle of compensation, how to perform compensation, the types of controls recommended and their use, basic strategies for designing a flow experiment, and data presentation.
http://vshow.on24.com/vshow/life/content/712257

Pluripotent stem cell protocols

View a selection of protocols for culturing, reprogramming, differentiating, and characterizing PSCs.
http://vshow.on24.com/vshow/life/content/720753

Frequently asked questions for the Molecular Probes® Alkaline Phosphatase Live Stain

Answers to commonly asked questions regarding the Molecular Probes® Alkaline Phosphatase Live Stain.
http://vshow.on24.com/vshow/life/content/720754

Vitronectin Essential 8™ Medium EDTA PSC코팅 플레이트에 든에서 로 계대배양법

Vitronectin 코팅 플레이트에 든Essential 8™ Medium에서 EDTA로 PSC 계대배양법
http://vshow.on24.com/vshow/life/content/720476

Qubit® 2.0: A Researchers Story

Interview with Tom Titus at the University of Oregon discussing the benefits of the Qubit 2.0 Quantitation platform for obtaining accurate DNA concentrations for his next gen sequencing experiments
http://vshow.on24.com/vshow/life/content/719815

Dr. Alexander Meissner, ISSCR 2013 -- TaqMan® hPSC Scorecard™ Panel

Dr. Meissner and Life Technologies have partnered to provide you with a new standard in pluripotent stem cell (PSC) characterization -- The TaqMan® hPSC Scorecard™ Panel enables verification of pluripotency and determination of lineage bias for both ES and iPS cell lines. In addition, the software allows you to characterize your stem cell lines relative to a standard.
http://vshow.on24.com/vshow/life/content/714116

Resource

http://vshow.on24.com/vshow/life/content/710616

A researcher's guide to RNA: RNA用户手册

A researcher's guide to RNA: RNA用户手册
http://vshow.on24.com/vshow/life/content/722537

EVOS FL Auto Flyer: EVOS® FL Auto全自动智能成像系统介绍

EVOS FL Auto Flyer: EVOS® FL Auto全自动智能成像系统介绍
http://vshow.on24.com/vshow/life/content/722534

ProFlex™ PCR System brochure

Learn more about the ProFlex™ PCR System.
http://vshow.on24.com/vshow/life/content/707851

Basics of flow cytometry, Part I: Gating and data analysis

An introduction to fluorescence, this presentation covers the following topics: the principle of the flow cytometry platform, the basic components of a flow cytometer, how to interpret a dye excitation/emission spectrum, how data is displayed, basic gating demonstration, and common statistics and terminology used in flow cytometry.
http://vshow.on24.com/vshow/life/content/712256

Genome editing of α-synuclein in iPSCs from a donor with multiple system atrophy

Presented ISSCR 2013
http://vshow.on24.com/vshow/life/content/720768

Qubit® Flyer

Qubit® 2.0 Fluorometer: the next generation of nucleic acid and protein quantitation
http://vshow.on24.com/vshow/life/content/711752

SuperScript® VILO™ Master Mix Product Bulletin

For improved qRT-PCR assay dynamic range
http://vshow.on24.com/vshow/life/content/711745

Cell biology product and resource guide, 2013

Complete list of all cell biology products and services offered by Life Technologies. Don't forget to check out the research areas for products tailored for your research.
http://vshow.on24.com/vshow/life/content/708034

Exosomes - frequently asked questions

Short video answers to questions about exosomes by Sasha Vlassov, Ph.D Senior Scientist R&D.
http://vshow.on24.com/vshow/life/content/710260

Resource

This webcast will provide you with an overview of how to be an effective booth representative. The 5 topics covered in this training are:
• Navigating the Virtual Environment • Who's Here Functionality • Group Chat & Private Chat Functionality • Updating the Attendee Profile • Sponsor Dashboard • Booth Representative Best Practices
http://vshow.on24.com/vshow/life/content/694852

An introduction to immunofluorescence staining of cultured cells

This webinar discusses the steps of an immunofluorescence staining protocol including: - Building a materials list - Review of common protocol variations - Addition of necessary controls Other topics covered include a simple troubleshooting guide, and how to avoid common pitfalls.
http://vshow.on24.com/vshow/life/content/712252

How to use the TaqMan® hPSC Scorecard™ Panel

This video will show you how to prepare and run the TaqMan® hPSC Scorecard™ Panel plates.
http://vshow.on24.com/vshow/life/content/714117

Genome editing with GeneArt® Precision TALs

Jon talks about the TAL effector proteins that were first identified as bacterial plant pathogens. Based on this, Life Technologies developed a new and powerful way to edit genomes and control gene function. The service GeneArt® Precision TALs provide custom DNA-binding proteins fused to a range of customer-designated effector domains that enable you to target the genome exactly where you want high precision and flexibility.
http://vshow.on24.com/vshow/life/content/719240

Use of surrogate reporter vectors to enrich for CRISPR & TALEN modified cells

Presented ISSCR 2013
http://vshow.on24.com/vshow/life/content/720769

AlexaFluor® secondary antibodies flyer

Learn more about AlexaFluor® secondary antibodies.
http://vshow.on24.com/vshow/life/content/710057

Modeling neurodegenerative disease - Parkinson’s disease cell models – White paper series 1

Modeling neurodegenerative disease - Stem cells help develop cellular models for understanding Parkinson’s disease - White paper series 1.
http://vshow.on24.com/vshow/life/content/719288

What is an Exosome (Episode 1)

Published on Oct 15, 2013 Episode 1 in a 6-part mini documentary series, see the full series here: http://www.lifetechnologies.com/exoso... We asked 10 prominent scientists to share their thoughts on science and in particular the field of exosomes research. The video-series tell the story and history of this exciting new area of research, and its impact on other research fields such as cancer and immunology. The video-series also discusses the potential future therapeutic and diagnostic applications that may come from exosome research.
http://vshow.on24.com/vshow/life/content/710240

Resource

http://vshow.on24.com/vshow/life/content/711858

Resource

http://vshow.on24.com/vshow/life/content/694851

Meet the inventor- TALs

This is an interview with Dr Jon Chesnut Life Technologies where he discusses his teams scientific discoveries and inventions that lead to products from Life Technologies.
http://vshow.on24.com/vshow/life/content/719242

ABCC1 gene disruption with TAL-protein genetic manipulation tool in the A549 human cell line

Presented 2012
http://vshow.on24.com/vshow/life/content/715934

Quantitate RNA with the Qubit® 2.0 Fluorormeter

The Qubit® 2.0 Fluorometer quantitates DNA, RNA, and protein with unprecedented accuracy, sensitivity, and simplicity. It is designed for molecular biology labs that work with precious samples that are rare or difficult to process or applications requiring precise measurement such as real-time PCR. More accurate than UV absorbance readings, Higher sensitivity - uses as little as 1 µL of sample, An intuitive, integrated system - sophisticated quantitation in 5 minutes or less
http://vshow.on24.com/vshow/life/content/719809

A different path to neural differentiation—Gibco® PSC Neural Induction Medium flyer

View workflow for neural induction which does not require rosettes or embryoid body formation. Learn about the new Gibco® PSC Neural Induction Medium.
http://vshow.on24.com/vshow/life/content/711967

Booth Training

http://vshow.on24.com/vshow/life/content/707352

Resource

http://vshow.on24.com/vshow/life/content/710610

Resource

http://vshow.on24.com/vshow/life/content/711792

Novel alkaline phosphatase live stain to identify and isolate emerging iPSCs

Presented ISSCR 2012
http://vshow.on24.com/vshow/life/content/710058

Stem cell products and services resource guide

View workflow examples, protocol overviews, and details about products and services available for stem cell research.
http://vshow.on24.com/vshow/life/content/711966

Advances in stem cell research: Essential 8™ Medium, a new standard for iPS cell culture

Dr. Emile Nuwaysir, Chief Operating Officer of Cellular Dynamics International, discusses the impact of variability in existing feeder-free culture media and how Essential 8™ feeder-free cell culture media solves these issues.
http://vshow.on24.com/vshow/life/content/717362

Modeling neurodegenerative disease - Parkinson’s disease cell models – White paper series 1

Modeling neurodegenerative disease - Stem cells help develop cellular models for understanding Parkinson’s disease - White paper series 1.
http://vshow.on24.com/vshow/life/content/719291

How does Sendai Virus reprogramming cells? 仙台病毒如何重编程细胞

How does Sendai Virus reprogramming cells? 仙台病毒如何重编程细胞
http://vshow.on24.com/vshow/life/content/722525

EVOS Brochure: EVOS 系列产品手册

EVOS Brochure: EVOS 系列产品手册
http://vshow.on24.com/vshow/life/content/722535

Stem cell products and services resource guide

View workflow examples, protocol overviews, and details about products and services available for stem cell research
http://vshow.on24.com/vshow/life/content/707688

Vitronectin PSC을 이용한 배양 플레이트 코팅 방법

이 비디오는 vitronectin을 이용한 배양 플레이트 코팅 방법을 알려드려 새로 만들어진 iPSC 콜로니나 동결 배양액을 Essential 8™ Medium으로 옮길 수 있게 합니다. Essential 8™ Complete Medium을 사용하기 전에, vitronectin로 코팅된 플레이트를 준비해야 합니다. Vitronectin의 최적 작업 농도는 세포주마다 다르기 때문에 경험적으로 판단해야 합니다. 세포 표면 최종 코팅 농도 0.1--1.0 μg/cm2 범위에서 세포주에 따라 선택하길 권장합니다. 우리는 인간 PSC 배양에 vitronectin 0.5 μg/cm2을 많이 사용합니다. 배양 용기 코팅 전에 다음 공식을 이용해 vitronectin 작업 농도를 계산하고 원액(stock)을 적절히 희석합니다. vitronectin 제품 설명서에 필요한 배양 접시 표면적과 vitronectin 양이 목록으로 나와 있습니다. 예를 들어 6 웰 플레이트를 0.5 μg/cm2 농도로 코팅하려면 희석액 6mL를 준비해야 합니다(웰 표면적 10cm2, 웰 당 희석 vitronectin 1 mL). vitronectin을 수령하면 실온에서 바이알을 해동하고 폴리프로필렌 시험관에 60μL씩 분주합니다. 이를 --80°C에서 얼리거나 즉시 사용합니다. 6-웰 플레이트의 웰을 코팅하기 위해 -80°C로 보관하고 있던 vitronectine 60μL를 꺼내 실온에서 해동합니다. 6-웰 플레이트당 60μL가 필요합니다. 해동한 vitronectin 60 μL를 실온에서 칼슘과 마그네슘이 없이 무균 DPBS 6mL가 들어있는 50-mL 원뿔형 시험관에 넣습니다. vitronectin 희석액을 피펫으로 위아래로 빨아오렸다 내렸다 하며 부드럽게 재현탁합니다. 이로 작업 농도 5 μg/mL, 즉 1:100 희석액이 만들어졌습니다. Add 1 mL of the 희석한 vitronectin 희석액 1mL를 6-웰 플레이트의 각 웰에 넣습니다. 웰당 1mL로 6-웰 플레이트(10 cm2/well)를 코팅하여 최종 농도가 0.5 μg/cm2이 되게 합니다. 다른 종류의 배양 용기를 사용한다면 양에 대한 자세한 정보는 vitronectin 제품 설명서를 참조하십시오. 이렇게 코팅한 플레이트를 실온에서 1시간 배양합니다. 참고: 이 배양 용기는 이제 사용하거나 실험실 필름으로 감싸 2--8°C에서 보관합니다. 용기가 건조해지지 않게 하며 사용 전 배양 용기를 1시간 이상 실온에 두어 온도를 높입니다.
http://vshow.on24.com/vshow/life/content/720485

Pluripotent Stem Cell Essential 8™ Medium 배양을 위한 완전한 준비 방법

이 비디오에서는 feeder없이 PSC 배양을 위한 완전한 Essential 8™ Medium 준비 방법을 알려드리고자 합니다. Essential 8™ Medium는 보충액이 필요없는(feeder - free) 성분이 완전히 조제된 배지로 인간 만능 줄기세포(PSC) 성장과 확장에 맞게 만들어졌습니다. 이는 원래 James Thomson 실험실에서 E8 Medium로 개발되었던 제품으로 Cellular Dynamics International에서 검증하였습니다. Essential 8™ Supplement (50X)를 하룻밤 동안 2--8°C에서 해동합니다. 37°C에서 해동하면 안됩니다. Essential 8™ Medium 500mL를 조제하려면 다음 성분을 무균적상태에서 혼합합니다. DMEM/F-12 (HAM) 1:1 490 mL Essential 8™ Supplement (50X) 10 mL 완성된Essential 8™ Medium는 2-8°C에서 최대 2주간 보관할 수 있습니다. 사용하기 전에 그 날 필요한 배지를 모두 실온에 두어 만져서 차갑게 느껴지지 않을 정도까지온도를 높입니다.배지를 37°C에서 따뜻하게 하면 안됩니다.
http://vshow.on24.com/vshow/life/content/720472

Platinum® Multiplex PCR Master Mix webinar

Join us for this previously recorded event. View at your leisure. Tired of investing so much time in optimizing your multiplex PCR reactions? Want to know more about what multiplexing can do for your throughput?
http://vshow.on24.com/vshow/life/content/711789

Modeling neurodegenerative disease - Parkinson’s disease cell models – White paper series 1

Modeling neurodegenerative disease - Stem cells help develop cellular models for understanding Parkinson’s disease - Series 1 white paper.
http://vshow.on24.com/vshow/life/content/719280

How to adapt pluripotent stem cells from other PSC media into Essential 8™ Medium

This video will show you how to adapt PSCs adapted to feeder-based or feeder-free media to Essential 8™ Medium and Vitronectin.
http://vshow.on24.com/vshow/life/content/717358

E-Gel® Imager system flyer

The E-Gel® Imager System makes agarose gel documentation and analysis personal. Now you can get the power and features of a larger and more complex gel imaging system combined with the sleek footprint and affordability of a benchtop device.
http://vshow.on24.com/vshow/life/content/711425

Protocols for reprogramming PSCs

View a selection of protocols for reprogramming PSCs from different cell types including PBMCs, CD34+, and fibroblasts.
http://vshow.on24.com/vshow/life/content/711817

Qubit® 2.0 Fluorometer: Making Sequencing “work”

Interview with Jason Carriere of the University of Oregon. He uses the Qubit platform to quantitate his nucleic acid samples before running next generation sequencing applications. "Things just work" for Jason when he starts with accurately quantitated samples by the Qubit fluorometer. Hear his story.
http://vshow.on24.com/vshow/life/content/719813

Quantitation of proliferating cells with the EVOS® FL Auto Cell Imaging System application note

Application note
http://vshow.on24.com/vshow/life/content/710557

Resource

http://vshow.on24.com/vshow/life/content/711874

Off-the-shelf GMP-compliant stem cells - human NSC and MSC flyer

Learn more about human NSC and MSCs available in an off-the-shelf, GMP-compliant format.
http://vshow.on24.com/vshow/life/content/710039

GeneArt® Precision TALs flyer - JAPANESE VERSION

Learn more about GeneArt® Precision TALs
http://vshow.on24.com/vshow/life/content/721111

Collecting Z-stack image sequences on the EVOS® FL Auto Cell Imaging System

Application note
http://vshow.on24.com/vshow/life/content/710559

Exosomes Brochure

Tools and technologies for exosome research
http://vshow.on24.com/vshow/life/content/707733

PSC Essential 8 Medium Vitronectin를 와 에 적응시키는 방법

PSC를 Essential 8 Medium와 Vitronectin에 적응시키는 방법
http://vshow.on24.com/vshow/life/content/720479